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KMID : 0614619940260050816
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Korean Journal of Gastroenterology
1994 Volume.26 No. 5 p.816 ~ p.824
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Studies on Mechanism of Elevated Serum gamma-Gultamyl Transpeptidase Activity During Chronic Hepatic Injuries in the Rat
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Abstract
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The mechanism of the elevation of serum γ-GTP activities by biochemical and enzyme histochemical methods on the experimentally induced extrahepatic cholestasis and hepatic septal fibrosis in rat. The animals with chronic extrahepatic cholestasis
induced by ligation of common bile duct were sacrificed at every 1 week for 4 weeks. The animals with septal fibrosis induced by biweekly intraperitoneal injection of swine serum were sacrificed at every 2 weeks for 12 weeks. The serum γ-GTP
activity
of both groups of animal was investigated by biochemical method and the obtained data were analyzed statistically. The livers of both animal groups were examined histopathologically and histochemically.
In animals with extrahepatic cholestasis, the activities of serum γ-GTP, AST, and ALT were elevated significantly than those of control. The elevation of serum enzyme activity was more marked in γ-GTP than others. In animals with hepatic septal
fibrosis, serum γ-GTP activity was slightly elevated than that of control but statistically not significant. The activities of serum AST and ALT were significantly elevated than those of control. The remarkable histological findings in the liver
with
ligation of common bile duct was marked proliferation of intraheaptic bile duct extending through hepatic lobules with time. the noticeable findings in the liver with administration of swine serum was septal fibrosis progressing more markedly and
more
frequently with time trend. In the animals with ligation of common bile duct or administration of swine serum, tissue γ-GTP activity in the liver was detected predominantly in epithelium of the bile ducts and lightly in the hepatocytes located
in
periportal areas.
On the basis of above findings it is suggested that elevation of serum γ-GTP activity is of biliary cell origin and not caused by hepatocyte induction, and that the induced γ-GTP from biliary cells flows back ward into sinusoidal blood.(Korean
J
Gastroenterol 1994 ; 26 : 816-824).
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